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(A) Location of mutated residues.
(B) Affinities (KA) of WT and mutant DrrA321–647 for PI(4)P-containing LUVs measured by SPR (left) and soluble PI(4)P analogs measured by ITC (right). Values and error bars are mean and SD (n = 2–4). arb. units, arbitrary units.
See also Figure S1B and Tables S2 and S3.
