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. Author manuscript; available in PMC: 2015 Mar 4.
Published in final edited form as: Structure. 2014 Feb 13;22(3):397–408. doi: 10.1016/j.str.2013.12.018

Figure 5. Biphasic Surface Pressure Responses of DrrA in Monolayer Insertion.

Figure 5

(A) Biphasic insertion of DrrA is indicated by a slope change in a Πe versus Πi plot. Inset: insertion time courses at different Πis; dotted lines are baseline corrected.

(B) The same data set was recast into a ΔΠ versus Πi plot and fitted with a biphasic insertion model (see text).

(C) Monolayer insertion of WT DrrA and the K568A and L610A/614A/615A (3LA) mutants with and without 20 mol % PI(4)P in the lipid monolayer.

(D and E) Monolayer insertion of DrrA at the indicated mole fractions of PI(4)P (D) and PI(4,5)P2 (E).

(F) Normalized Ca2+-inhibited DrrA insertion at different Πis overlaid with Ca2+ affinity measurements determined by surface pressure titration. Inset: insertion time courses of DrrA at different Ca2+ concentrations. Values and errors bars represent mean and SD for two to four independent measurements.