FIGURE 2.

Characterization of HDL proteins after guanidine treatment of HDL in the presence or in the absence of PP-PLA₂. A, Gd-HDL and Gd-PP-PLA₂-HDL were separated by SEC into two and three fractions, respectively. SEC conditions were the same as described in the legend to Fig. 1. B, NDGGE of Gd-HDL and Gd-PP-PLA₂-HDL SEC fractions. Total lanes represent Gd-HDL and Gd-PP-PLA₂-HDL before SEC separation. For NDGGE analysis, 3 μg of protein was loaded per lane. Molecular weight standards are the High Molecular Weight Calibration Kit (GE Healthcare). C, SDS-PAGE of Gd-HDL SEC fractions. D, SDS-PAGE of Gd-PP-PLA₂-HDL SEC fractions. For SDS-PAGE analysis, 5 μg of protein was loaded per lane. Molecular weight standards are Precision Plus Protein Dual Xtra standards (Bio-Rad). a.u., absorbance units.