TABLE 3.
LC-MS and MALDI-TOF MS analysis of control-apoA-I and PP-PLA2-apoA-I after SDS-PAGE separation
The gel bands numbered 1–6 in Fig. 4B were excised from the gel and digested with trypsin, and the tryptic digest was analyzed by LC-MS. The sequence of the tryptic peptides identified by LC-MS is highlighted in red. In a parallel SDS-PAGE experiment, the mass of the main protein component in undigested samples from the same gel bands was determined by MALDI-TOF MS (see “Experimental Procedures” and Table 1) and is reported in parenthesis. The sequence of the apoA-I peptide with calculated mass closest to the mass of the undigested protein fragment, as determined by MALDI-TOF, is shown in boldface type.
