FIGURE 7.

The ET axis is involved in the MφCM-induced transendothelial migration of MCF-7 cells. A, schematic experimental design. The detailed procedure is described under “Experimental Procedures.” B, representative photographs of MCF-7 cells that had migrated through the endothelial cell layer under different experimental conditions (magnification, ×100). C, quantitative data from the transendothelial migration assay. It was observed that MφCM induced more MCF-7 cells to pass through the endothelial cell layer. This phenomenon could be inhibited by 100 ng/ml BQ123 or BQ788. The data represent the mean ± S.D. (error bars) of three independent experiments. a, p < 0.05 compared with control medium (CTRL) plus PBS. b, p < 0.05 compared with MφCM plus PBS.