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FLJ00018 is activated through H-Ras. A and B, NIH3T3 cells were co-transfected with expression vectors for FLJ00018 (F018), EGFR, and a constitutively active form of H-Ras (H-RasCA) (A) or a dominant-negative form of H-Ras (H-RasDN) (B) as indicated. Transfected cells were stimulated with 20 ng/ml EGF for 15 min. Equal amounts of the proteins were resolved by 7.5% SDS-PAGE. To detect Myc-tagged FLJ00018, immunoblotting was performed with antibodies against Myc.
