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. 2014 Feb 19;289(14):10069–10083. doi: 10.1074/jbc.M113.535351

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — PHF6-ePHD2 domain interacts with dsDNA. A and B, FPA of PHF6(208-333) (A) and PHF6(208-365) (B) interaction with dsDNA oligonucleotides with different lengths. Protein PHF6(208-333) and PHF6(208-365) concentrations are as follows, respectively: 0, 1.45, 2.9, 5.8, 11.6, 23.2, 46.4, 58, 74.25, 87; 0, 0.65, 1.3, 2.6, 5.2, 10.4, 20.8, 39, 52, 65, and 78 μm. C and D, FPA of PHF6(208-333) (C) and PHF6(208-365) (D) interaction with AT-rich and GC-rich dsDNA. Protein PHF6(208-333) concentrations are same as A. Protein PHF6(208-365) concentrations are as follows: 0, 0.65, 1.3, 2.6, 5.2, 10.4, 26, 39, 52, 65, and 78 μm. E and F, EMSA of GST-PHF6(208-333) (E) and GST-PHF6(208-365) (F) interaction with 33 bp of GC-rich dsDNA (2 μm) in 5% native gel. Protein GST-PHF6(208-333) and GST-PHF6(208-365) concentrations are as follows, respectively: 15.4, 30.8, 61.7, 123.4, 185, 247, 14.2, 28.3, 56.7, 113.4, 226.8, and 283.5 μm.