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Kd value (μm) of PHF6-ePHD2 domain binding to different types of DNA
| DNA type | PHF6(208-333) | PHF6(208-365) | R209E/K211E | K234E/K235E | K273E/R274E/K276E/R277E | K276E/R277E/M278A/K279E |
|---|---|---|---|---|---|---|
| 14 bp | 12.84 ± 0.88 μm | 7.26 ± 0.71 μm | 62.03 ± 16.84 μm | No binding | No binding | No binding |
| 18 bp | 16.54 ± 1.56 μm | 7.92 ± 0.70 μm | ||||
| 20 bp | 16.44 ± 1.60 μm | 7.09 ± 0.58 μm | ||||
| AT-rich | 20.51 ± 1.93 μm | 4.15 ± 0.55 μm | ||||
| GC-rich | 30.54 ± 2.34 μm | 5.24 ± 0.66 μm |
