FIGURE 6.

Inhibition of Src, PI3-kinase, or mTORC1 blocks the tTG-enhanced cell survival. A and B, NIH3T3 cells stably expressing the vector alone or Myc-tagged tTG were placed in serum-free medium supplemented without (not treated; NT) or with PP2, LY294002, MDC, or rapamycin for 36 h, at which time the cells were collected and stained with DAPI to identify condensed and/or blebbed nuclei. Percent apoptosis was determined by calculating the ratio of apoptotic to non-apoptotic cells. The experiments were performed in triplicate, and the results were averaged. The error bars indicate standard deviation, and the p values determined for the different conditions are as follows; *, p < 0.05; **, p < 0.01; ***, p < 0.001.