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The M26R, W94A, and K85E GCAP1 compete with wild type in theRetGC1 assay. RetGC1 activated half-way by 1 μm WT GCAP1 in the presence of 10 mm Mg2+ and 2 mm EGTA was assayed in the presence of increasing concentrations of the following GCAP1 mutants: M26R (○); F73E (●); P30Y (♢); Y37R (■); K85E (♦); R93E (□); and W94A (▴), empirical curve fit.
