TABLE 2.
GCAP1 | K1/2 GCAP | amax |
---|---|---|
WT | 1.6 ± 0.1 | 27 ± 1.6 (n = 5) |
E17K | 8.3 ± 0.1 | 18 ± 0.3 (n = 2) |
C18T,C29T,C106T,C125T | 12 ± 2 | 29 ± 1 (n = 3) |
C18T,C106T,C125T | 1.2 ± 0.2 | 25 ± 0.2 (n = 3) |
H19R | 4.5 ± 0.4 | 18 ± 1 (n = 2) |
K23D | 25 ± 4a | 9 ± 0.9 (n = 2) |
M26R | ND | ND (n = 2) |
T27K | 3.3 ± 0.5 | 28 ± 2 (n = 2) |
T27E | 2.9 ± 0.1 | 28 ± 0.4 (n = 2) |
E28R | 29 ± 4a | 28 ± 3 (n = 2) |
P30Y | ND | ND (n = 5) |
S31Y | 60 ± 19a | 10 ± 3 (n = 2) |
G32N | 13 ± 3a | 39 ± 12 (n = 2) |
K46D | 9.7 ± 0.9 | 31 ± 1.4 (n = 2) |
F73E | ND | ND (n = 2) |
M74K | 7.1 ± 0.5 | 17 ± 1 (n = 2) |
V77E | ND | ND (n = 3) |
A78E | ND | ND (n = 3) |
K85E | ND | ND (n = 2) |
K87D | 15 ± 1 | 19 ± 0.4 (n = 2) |
W94A | ND | ND (n = 2) |
K97S | 13 ± 1 | 12 ± 1 (n = 2) |
S141Y | 1.6 ± 0.1 | 27 ± 0.4 (n = 2) |
S152E | 7.2 ± 0.6 | 20 ± 0.6 (n = 2) |
L153R | 3.2 ± 0.1 | 27 ± 0.5 (n = 2) |
L166R | 3.9 ± 0.2 | 28 ± 0.7 (n = 2) |
a Because of the dramatic loss of affinity for the target and limitations of the fit method, the error was larger for those mutants in which K1/2 GCAP was similar to or above the maximal GCAP concentration in the assay, and the measured a was much lower than amax.