FIGURE 4.
Effects of extracellular myocilin on cell proliferation. A, the WST-1 proliferation assay. HEK293 cells were treated with CM from Tet-On cells expressing (+ Myocilin) or non-expressing (− Myocilin) myocilin and tested by the WST-1 proliferation assay after 48 h. To deplete myocilin in CM, it was preincubated with monoclonal anti-myocilin antibodies (5 μg/ml) for 2 h. As a control, CM was preincubated with mouse control immunoglobulin for 2 h. B, 1 × 105 cells/well were incubated as in A and counted after 48 h (*, p < 0.05). Error bars, S.D. of triplicate cultures.