Characterization of wild-type and Myoc-null mice MSCs identities by analysis of MSC marker expression. Mouse MSCs were stained with the indicated primary antibodies supplied with the mouse multipotent mesenchymal stromal cell marker antibody panel (R&D Systems). MSCs were stained with a phycoerythrin-conjugated goat anti-rat secondary antibody and analyzed by flow cytometry. Wild-type (middle panels) and Myoc-null (right panels) cells demonstrate the expected phenotype for mouse MSCs (positive expression of Sca-1, CD106, CD73, CD29, and CD44; negative expression of CD45 and CD11b). The left panels correspond to unstained MSCs (top) and staining with anti-rat secondary antibody (bottom), respectively.