FIGURE 1.

The cuf1⁺ gene is required for copper starvation-dependent induction of ctr4⁺ and ctr5⁺ transcripts during meiosis. Representative expression profiles of ctr4⁺ and ctr5⁺ transcripts in pat1-114/pat1-114 cuf1⁺/cuf1⁺, and pat1-114/pat1-114 cuf1Δ/cuf1Δ cells that were induced to undergo synchronous meiosis. Cells were either left untreated (−) or incubated in the presence of TTM (50 μm) or CuSO₄ (50 μm). At the indicated times after meiotic induction, ctr4⁺, ctr5⁺, and act1⁺ (internal control) mRNA levels were analyzed in control strain (cuf1⁺/cuf1⁺) (panels A and C) and isogenic strain lacking the cuf1⁺ alleles (cuf1Δ/cuf1Δ) (panels B and D).