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. 2014 Feb 26;289(14):10182–10191. doi: 10.1074/jbc.M113.533489

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Dab1 and Akt phosphorylation in cultivated chicken granulosa sheets. A, granulosa sheets from F1 follicles were stimulated with MCM or RCM (recombinant mouse Reelin expressed and secreted by 293 HEK cells) for 30 min, and RIPA lysates were analyzed by Western blotting. Phosphorylated Dab1 was detected with a rabbit polyclonal anti-p-Dab1 (Tyr-220) antibody, and detection of total Dab1 levels was carried out with a mouse monoclonal anti-chicken Dab1 antibody (6H9-1A4). B, the same lysates were used for the determination of Akt phosphorylation. A rabbit polyclonal anti-p-Akt (Ser-473) antibody was used to detect phosphorylated Akt. Total Akt levels were detected with a rabbit polyclonal anti-Akt antibody.