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. 2014 Feb 20;289(14):10211–10222. doi: 10.1074/jbc.M113.506246

FIGURE 1.

FIGURE 1.

HPβCD treatment results in activation of TFEB and the CLEAR network. A, confocal microscopy analysis of TFEB subcellular localization in HeLa cells stably transfected with TFEB-FLAG treated with 0.1, 1, and 10 mm HPβCD or sucrose 100 mm for 1 day. Co-localization of DAPI (blue, first column) and an anti-FLAG antibody (red, first column) is shown in purple (third column). The scale bar is 10 μm. UT, untreated; Sucr, sucrose. B, percentage of cells presenting nuclear localization of TFEB-FLAG upon treatment with HPβCD (0.1, 1, and 10 mm) or sucrose (100 mm). Representative (∼30) fields containing ∼50 cells were analyzed (p < 0.05; *, p < 0.01). Data are reported as the mean ± S.D. C, relative mRNA expression levels of representative genes of the lysosome system in HeLa cells stably transfected with TFEB-FLAG treated with 1 mm HPβCD for 1 day. mRNA expression levels of GBA, HEXA, LAMP1, were obtained by qRT-PCR, corrected by the expression of the house-keeping gene GAPDH, and normalized to those of untreated cells (dashed line). Data are reported as the mean ± S.D. n ≥ 3; p < 0.05.