FIGURE 1.
Chromatographic separation and determination of mass isotopomer distribution of long-chain acylcarnitines and acyl-CoAs extracted from [1,2,3,4-13C4]palmitate-perfused working rat heart. Shown is a representative chromatogram showing the UHPLC separation and mass isotopomer distribution of the carnitine and CoA derivatives of myristic, palmitic, and stearic acid extracted from rat heart perfused for 60 min with [1,2,3,4-13C4]palmitic acid (M+4). In the insets, the most abundant isotopomer was set to 100%.