FIGURE 2.
Chromatographic separation and determination of mass isotopomer distribution of short-chain acylcarnitines and acyl-CoAs extracted from [1,2,3,4-13C4]palmitate-perfused working rat heart. Shown is a representative chromatogram showing the UHPLC separation and mass isotopomer distribution of the acetyl- and malonylcarnitine and their respective CoA derivatives extracted from rat heart perfused for 60 min with [1,2,3,4-13C4]palmitic acid (M+4). In the insets, the most abundant isotopomer was set to 100%.