TABLE 1.
Effect of perfusion of rat heart with palmitate on cardiac acylcarnitine profile
Total, free, and individual short-, medium-, and long-chain acylcarnitines were quantified in rat hearts at baseline (no perfusion) and after 60-min perfusion in the working mode with unlabeled palmitic acid. The numbers represent the mean ± S.E. of six (baseline) and four (perfusion) separate experiments. ND, not determined.
| Acylcarnitine species | Heart acylcarnitines |
|
|---|---|---|
| Baseline (no perfusion) | Perfusion with palmitate | |
| nmol/g wet weight | nmol/g wet weight | |
| Total carnitine | 873.8 ± 150.3 | 579.9 ± 78.5 |
| Free carnitine | 563.2 ± 124.1 | 304 ± 15.8 |
| Acylcarnitines | 310.62 ± 64.4 | 276.0 ± 94.0 |
| Acetyl | 260.6 ± 63.6 | 62.7 ± 3.7a |
| Butyryl | 1.7 ± 0.8 | ND |
| Isobutyryl | 0.3 ± 0.4 | ND |
| (R)-3-Hydroxybutyryl | 2.3 ± 0.3 | ND |
| (S)-3-Hydroxybutyryl | 6.7 ± 2.5 | 1.6 ± 0.07 |
| 2-Hydroxyisovaleryl | 0.7 ± 0.5 | ND |
| 2-Methylbutyryl | 0.3 ± 0.4 | ND |
| Lauroyl | ND | 1.8 ± 1.2 |
| Myristoyl | 0.75 ± 0.67 | 15.4 ± 10.2a |
| (S)-3-hydroxymyristoyl | ND | 0.7 ± 0.85 |
| Palmitoyl | 2.81 ± 0.98 | 135.9 ± 40.7a |
| (S)-3-Hydroxypalmitoyl | 0.2 ± 0.25 | 6.0 ± 2.9a |
| Stearoyl | 1.11 ± 0.65 | 16.34 ± 9.8a |
| (S)-3-Hydroxystearoyl | ND | 0.6 ± 0.7 |
| Oleyl | 1.82 ± 0.8 | 1.5 ± 0.92 |
| Linoleyl | 2.08 ± 0.8 | 1.6 ± 0.99 |
| Malonyl | 3.3 ± 0.65 | 2.0 ± 0.35 |
| Succinyl | 7.2 ± 1.6 | 5.9 ± 1.06 |
a Carnitine species whose content showed a significant increase or decrease (p < 0.05, Student's t test) after perfusion.