FIGURE 6.

Co-treatment of cells with Dex plus GnRH synergistically enhances SGK-1 mRNA levels. LβT2 cells were incubated for 8 h in serum-free medium with 100 nm Dex, 100 nm GnRH, or a combination of both. Total RNA was isolated and reverse-transcribed, and relative mRNA levels of several endogenous GRE-containing genes including GILZ (A), MKP-1 (B), FKBP5 (C), FSHβ (D), and SGK-1 (E) were determined by quantitative real-time PCR. Fold changes in the gene of interest mRNA levels were normalized to GAPDH transcripts and were calculated relative to vehicle-treated (control (Ctrl)) samples. The graph is representative of three independent experiments. One-way ANOVA with Dunnett's (stars above bars) and Tukey (stars above lines joining bars) post tests were used for statistical analysis and denoted as *, **, or *** to indicate p < 0.05, p < 0.01, or p < 0.001, respectively.