FIGURE 8.

Synergism involves differential recruitment of SRC cofactors compared with Dex and GnRH alone without an increase in GR recruitment to the SGK-1 promoter. ChIP assays were performed in LβT2 cells that were treated for 1 h with 100 nm Dex, 100 nm GnRH, and both together using anti-GR (A), anti-SRC-1 (B), anti-GRIP-1 (C), and anti-SRC-3 (D) antibodies. Precipitated complexes that were bound to the SGK-1 promoter were detected with quantitative real-time PCR using primers that span the GRE region. Results were normalized against the input samples and are represented relative to control. The graphs are representative of three independent experiments. One-way ANOVA with Dunnett's post-test was used for statistical analysis and is denoted as *, **, or *** to indicate p < 0.05, p < 0.01, or p < 0.001, respectively.