FIGURE 2.

Transduced TAT-Neph1CD inhibited tyrosine phosphorylation of endogenous Neph1. A, podocytes transduced with either TAT-GFP or TAT-Neph1CD were treated with PAN for a period of 48 h, and the cell lysates were analyzed for the presence of phosphorylated Neph1 by Western blotting (WB) with phospho-Neph1 (p-Neph1) and Neph1 antibodies. The transduction of TAT-Neph1CD in podocytes significantly reduced PAN-induced endogenous Neph1 phosphorylation as compared with the control. B, further densitometric analysis revealed a 60–70% decrease of the PAN-induced endogenous Neph1 phosphorylation in cells transduced with TAT-Neph1CD as compared with the TAT-GFP-transduced cells.