FIGURE 1.

Gene-targeting strategy and adipose-specific deletion of FIT2. A, graphical representation of the gene-targeting strategy used in this study to delete FIT2 in adipose depots. B, quantitative RT-PCR analysis of FIT2 mRNA in the indicated tissues shows adipose-specific deficiency of FIT2 in AF2KO mice. C, Western blot analysis of FIT2 on the indicated tissues shows adipose-specific deficiency of FIT2 protein in EWAT, SWAT, and BAT but not in the heart of AF2KO mice. D, quantitative RT-PCR of FIT2 mRNA in peritoneal macrophage from L/L and AF2KO mice (macrophage harvested from 4 mice/genotype). Data are represented as mean ± S.E. (error bars).