FIGURE 8.

TAG synthesis rates in differentiated WAT precursor cells in culture. WAT precursor cells were differentiated to day 6 and treated with 50 μm [¹⁴C]oleate-BSA complex for the times indicated, and radioactive TAG was quantified by TLC and phosphorimaging analysis. Basal TAG turnover (i.e. non-stimulated) was measured in WAT precursor cells differentiated to day 6. Cells were labeled overnight with 50 μm [¹⁴C]oleate-BSA and then washed and incubated in fresh medium without radiotracer for the times indicated. Radioactive cellular TAG was quantified by TLC and phosphorimaging analysis. Data are represented as mean ± S.D. (error bars) No significant differences were found.