FIGURE 10.
Effect of high O2 condition on differentiation efficiency of hiPSCs in D'Amour and Nostro protocol. A, scheme of the D'Amour differentiation protocol and the timeline of high O2 condition. Ex-4, exendin-4; HGF, hepatocyte growth factor; IGF1, insulin-like growth factor 1. B, values are the percentage of C-peptide-positive cells/well of the cells treated with high O2 condition during three different stages. *, p < 0.05 versus corresponding control. n = 6 each. C, levels of INS gene were analyzed on day 17 of differentiation in cells treated with high O2 condition during five different stages by qPCR. *, p < 0.05 versus corresponding control. n = 7 each. D, scheme of the Nostro differentiation protocol and the timeline of high O2 condition. E, values are the percentage of C-peptide-positive cells/well of the cells treated with high O2 condition during three different stages. n = 3 each. Error bars, S.E.