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. 2014 Feb 19;289(14):9623–9638. doi: 10.1074/jbc.M113.524363

FIGURE 4.

FIGURE 4.

Effect of high O2 condition on Notch signaling. A, scheme of the timeline of high O2 condition. B, levels of HIF-1α and β-actin (loading control) were analyzed on day 6 of differentiation in normoxia, high O2, or hypoxia (1% O2; positive control)-treated ING112 cells by immunoblotting. C–E, levels of Vegfa (C), Hes1 (D), and Ngn3 (E) gene were analyzed on days 3, 4, 5, and 6 of differentiation in normoxia or high O2-treated ING112 cells by qPCR. *, p < 0.05; **, p < 0.01 versus corresponding control. n = 8 each. F, levels of Vegfa, Hes1, and Ngn3 genes were analyzed on day 6 of differentiation in DMSO or 1 nm echinomycin-treated ING112 cells by qPCR. *, p < 0.05; **, p < 0.01 versus corresponding control. n = 3 each. Error bars, S.E.