FIGURE 1.

Striatin 3 coiled coil domain forms an asymmetric parallel dimer. A, organization of the STRIPAK complex. Striatins are novel PP2A B‴ subunits that interact with PP2A A and C subunits via their coiled coil domains, and bind GCKs via adaptors. B, overall structure of the SeMet-labeled striatin 3 coiled coil domain. Chains A and B of striatin 3 are depicted as helical ribbons and colored green and orange, respectively. The coiled coil domain forms an asymmetric dimer, in which chain B contains a large bend. Noncanonical residues in the coiled coil result in formation of the asymmetric dimer. C, size of bend of the coiled coil axis. D, analysis of the heptad repeats of the striatin 3 coiled coil. Residues that may be involved in asymmetry of striatin 3 coiled coil are colored red.