FIGURE 1.

Cell adhesion activity of recombinant NELL1. A and B, dose-dependent adhesion of MC3T3-E1 cells to full-length NELL1 (NELL1-FL) and fibronectin (FN). These proteins were coated onto 96-well plates at the indicated concentrations (Conc.). MC3T3-E1 cells were allowed to adhere for 1.5 h at 37 °C. Non-adherent cells were washed from the wells, and adherent cells were then fixed and stained with crystal violet. Representative images (A) and the number of adhered cells per area (B) are shown for cells adhering to NELL1-FL and fibronectin. C, kinetics of cell adhesion to NELL1-FL and fibronectin. NELL1-FL (100 nm) and fibronectin (10 nm) were coated onto 96-well plates, and MC3T3-E1 cells were allowed to adhere for the indicated times at 37 °C before the adhered cells were counted. Each value represents the mean ± S.E. of triplicate results.