FIGURE 10.

Integrin α3β1 mediates MC3T3-E1 cell adhesion to NELL1. NELL1-FL or NELL1-VWC345 was coated onto 96-well plates at a concentration of 100 nm. MC3T3-E1 cells were preincubated with function-blocking anti-integrin antibodies (5 μg/ml) for 15 min and then allowed to adhere for 1.5 h at 37 °C before the adhered cells were counted. Each value represents the mean ± S.E. of triplicate results relative to those of untreated cells set to 100%. ***p < 0.001 (Student's t test).