FIGURE 11.

FAK and ERK1/2 phosphorylation in MC3T3-E1 cells adhering to NELL1. A, serum-starved MC3T3-E1 cells were detached and treated with or without FAK inhibitor 14 (1 μm) for 30 min and then plated on dishes coated with 100 nm NELL1-FL, 100 nm NELL1-VWC345, or 0.01% poly-l-lysine (PLL) for 1.5 h. Cell lysates were analyzed by immunoblotting with anti-phosphorylated-FAK and anti-phosphorylated-ERK1/2 antibodies. Anti-FAK, anti-ERK1/2, and anti-β-actin blots were used as internal controls. B, serum-starved MC3T3-E1 cells were detached and plated on dishes coated with 100 nm NELL1-FL, 100 nm NELL1-mut2345, or 0.01% PLL for 1.5 h. Cell lysates were analyzed by immunoblotting with anti-phosphorylated-FAK and anti-phosphorylated-ERK1/2 antibodies.