FIGURE 11.

In vitro FGF-23 protein treatment of isolated BM Ter119+ cells normalizes erythropoiesis in Fgf-23^−/− mice (n = 3 per group). Cells from each mouse were cultured in triplicate. A, percentage of mature erythroid cells stained positive for Ter119. B, percentage of mature erythroid cells stained positive for Ter119 and Epo-R. C and D, quantitative real time RT-PCR showing mRNA levels of Epo (C) and HIF-2α (D) in WT and Fgf-23^−/− Ter119+ cells with or without FGF-23 protein treatment. The data are represented as mean fold change ± S.E. relative to housekeeping gene HPRT. *, p < 0.05; **, p < 0.01.