Skip to main content
. 2014 Feb 7;289(14):9795–9810. doi: 10.1074/jbc.M113.527150

FIGURE 12.

FIGURE 12.

Deletion of vitamin D rescues the hematopoietic phenotype of Fgf-23−/− mice. A–D, peripheral blood complete blood count analysis of 6-week-old WT, Fgf-23−/−, Fgf-23−/−/1α(OH)ase−/−, and 1α(OH)ase−/− mice. A, RBC counts (WT, n = 21; Fgf-23−/−, n = 6; Fgf-23−/−/1α(OH)ase−/−, n = 8; and 1α(OH)ase−/− n = 13). B, percentage of primitive pro-E-stained positive for Ter119med and CD71high (WT, n = 4; Fgf-23−/−, n = 4; Fgf-23−/−/1α(OH)ase−/− n = 5; and 1α(OH)ase−/− n = 9). C, percentage of mature erythroid cells stained positive for Ter119 and CD71low (Ery-C fraction) (WT, n = 8; Fgf-23−/−, n = 7; Fgf-23−/−/1α(OH)ase−/− n = 6; and 1α(OH)ase−/− n = 7). D, percentage of HSC population stained for SLAM (CD150+CD48−) (WT, n = 7; Fgf-23−/−, n = 9; Fgf-23−/−/1α(OH)ase−/− n = 7; and 1α(OH)ase−/− n = 8). E and F, FGF-23 injections. E, percentage of primitive pro-erythroblasts (pro-E) stained positive for Ter119med and CD71high (WT, n = 5; Fgf-23−/−, n = 6; Fgf-23−/−/1α(OH)ase−/− n = 3; and 1α(OH)ase−/− n = 3). F, percentage of mature erythroid cells stained positive for Ter119 (WT, n = 7; 1α(OH)ase−/− n = 12; WT+FGF-23, n = 6; 1α(OH)ase−/− + FGF-23, n = 3). The data are represented as means ± S.E. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 compared with WT; #, p < 0.05 compared with fgf-23−/−; ##, p < 0.01 compared with fgf-23−/−; $, p < 0.05 compared with fgf-23−/−/1α(OH)ase−/−; $$, p < 0.01 compared with fgf-23−/−/1α(OH)ase−/−.

HHS Vulnerability Disclosure