FIGURE 6.

Flow cytometry analysis of HSC populations in peripheral blood and bone marrow from 6-week-old WT and Fgf-23^−/− mice. A, percentage of HSC population in peripheral blood stained for SLAM (CD150⁺CD48⁻) (WT, n = 7; Fgf-23^−/−, n = 10). B, percentage of HSC population in bone marrow stained for SLAM (CD150⁺CD48⁻) (WT, n = 6; Fgf-23^−/−, n = 11). C, percentage of HSC population undergoing apoptosis in the bone marrow stained for SLAM (CD150⁺CD48⁻) and annexin V (WT, n = 6; Fgf-23^−/−, n = 11). D, colony-forming assay for granulocyte-erythrocyte-monocyte-megakaryocyte (CFU-GEMM) progenitors (WT, n = 5; Fgf-23^−/−, n = 12). Cells from each mouse were plated in duplicate, and the number of colonies in each plate was counted. The data are represented as means ± S.E. *, p < 0.05.