FIGURE 7.

Migration and homing of Fgf-23^−/− bone marrow cells. A, SDF-1α concentration measured by ELISA in serum of WT (n = 9) and Fgf-23^−/− mice (n = 8). Samples were measured in duplicate. B, in vitro migration experiment revealed no change in migratory capacity of Fgf-23^−/− BM cells toward an SDF-1 gradient (WT, n = 18; Fgf-23^−/−, n = 14). C, adherence assay showed no changes in Fgf-23^−/− BM cells to adhere to fibronectin (WT, n = 10; Fgf-23^−/−, n = 9). D, graphic representation of flow cytometry analysis of peripheral blood, bone marrow, and spleen (SPL) after transplantation of WT or Fgf-23^−/− BM cells in irradiated B6.SJL recipient mice (WT, n = 10; Fgf-23^−/−, n = 11). E–G, flow cytometry profile of peripheral blood, bone marrow, and spleen after transplantation. The data are represented as means ± S.E. *, p < 0.05; **, p < 0.01; ***, p < 0.001.