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. 2014 Feb 7;289(14):9795–9810. doi: 10.1074/jbc.M113.527150

FIGURE 9.

FIGURE 9.

Flow cytometry analysis and colony-forming assays of fetal liver cells from E15.5 WT and Fgf-23−/− mice. A and B, flow cytometry. A, percentage of mature erythroid cells stained positive for Ter119 (WT, n = 8; Fgf-23−/−, n = 8). B, colony-forming assay for erythroid (BFU-E) progenitors (WT, n = 10; Fgf-23−/−, n = 10). Cells from each mouse were plated in duplicate, and the number of colonies in each plate was counted. C, percentage of HSC population stained for SLAM (CD150+CD48−) (WT, n = 7; Fgf-23−/−, n = 8). D, representative flow cytometry dot plot showing CD150+CD48− populations in WT and Fgf-23−/− mice. E, percentage of HSC population stained for LSK (Lin−Sca-1+c-Kit+) (WT, n = 9; Fgf-23−/−, n = 10). F, representative flow cytometry dot plot showing LSK populations in WT and Fgf-23−/− mice. G, percentage of HSC population stained for KTLS (c-Kit+Thy1+Lin−Sca-1+) (WT, n = 9; Fgf-23−/−, n = 10). The data are represented as means ± S.E. *, p < 0.05; **, p < 0.01.

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