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Menin is required for processing of let-7a. Radiolabeled pri-let-7a was incubated with cell extracts from WT and menin-null MEFs, and the RNA was resolved on a 6% urea-polyacrylamide gel followed by autoradiography. The substrate, pri-let-7a, and product, pre-let-7a, are as indicated. nt, nucleotides.
