FIGURE 5.

Disulfide bond formation between P2X2 subunits after cysteine substitution. The oligomeric state of P2X2 subunits was determined after cysteine substitution in the turret region (A), the lower body wall (B), and the outer end of the TM domains (C). Measurements of band intensity for monomers (black bars), dimers (gray bars) and trimers (white bars) of each mutant were made from non-reducing blots. Values represent percentage of the total protein and are means ± S.E. from three independent experiments. In each case, the right side shows representative Western blots from individual experiments.