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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cancer Discov. 2013 Dec 17;4(3):362–375. doi: 10.1158/2159-8290.CD-13-0609

Figure 2. Sensitivity to ABT-199 positively correlates with endogenous BCL-2 protein level and negatively correlates with BCL-XL protein level in AML cell lines.

Figure 2

A) Western blot analysis of BCL-2 family proteins in untreated AML cells. The band intensity was quantified using Odyssey v2.0 software, and displayed numerically as a ratio of the band intensity detected in the OCI-AML3 cells. B) Significant correlations were observed between ABT-199 IC50 values and BCL-2/BCL-XL protein levels. The non-parametric one-tailed Spearman test was used to determine the correlation coefficient. The p values provided are nominal p values not corrected for multiple comparisons. C). MCL-1 knockdown by 85% was achieved by lentiviral shRNA. D). MCL-1 knockdown significantly sensitized OCI-AML3 cells to ABT-199. E). Western blot analysis showing HL-60 AML cells transfected to stably overexpress BCL-XL or BCL-2. F). Overexpression of BCL-XL or BCL-2 in HL-60 cells confers complete resistance to ABT-199-induced apoptosis.