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. 2014 Mar 28;8:88. doi: 10.3389/fncel.2014.00088

FIGURE 2.

FIGURE 2

The sensitivity of GABAARs to GABA in RFP-positive cells in the E17.5 cerebral cortex does not differ between wild-type and GAD67-GFP knock-in mice. (A) Representative GABA-evoked currents recorded in an RFP-positive cell from a GAD67+/+ fetus. The holding potential (VH) was -60 mV. The puff application of GABA was stopped immediately after desensitization was initiated. (B) Dose–response curves of GABA currents. The EC50s of the current responses in GAD67+/+, GAD67+/GFP, and GAD67GFP/GFP fetuses were 16.23 ± 1.75, 17.46 ± 2.11, and 19.22 ± 2.43 μM, respectively, in the CP/SP (P = 0.657 by ANOVA); 16.98 ± 1.80, 20.28 ± 1.13, and 18.53 ± 2.64 μM, respectively, in the IZ (P = 0.512); and 16.24 ± 2.61, 18.79 ± 1.66, and 20.39 ± 2.12 μM, respectively, in the SVZ/VZ (P = 0.422). The Hill coefficients were 1.36 ± 0.13, 1.26 ± 0.08, and 1.34 ± 0.07, respectively, in the CP/SP (P = 0.696); 1.49 ± 0.11, 1.28 ± 0.10, and 1.28 ± 0.08, respectively, in the IZ (P = 0.295); and 1.33 ± 0.11, 1.28 ± 0.10, and 1.23 ± 0.16, respectively, in the SVZ/VZ (P = 0.891). Error bars: mean ± SEM.