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. Author manuscript; available in PMC: 2015 Jan 30.
Published in final edited form as: Cell. 2014 Jan 30;156(3):590–602. doi: 10.1016/j.cell.2013.12.041

Figure 6. CTX-dependent secretory responses can be elicited in independent murine tumor models, patient derived cells and independent effector cells.

Figure 6

(A) A bar graph showing macrophage-dependent phagocytosis of in vivo CTX treated murine Arf−/−Ph+ B-ALL in the absence or presence of the 18B12 anti-CD20 antibody. (B) A bar graph showing the effect of conditioned media generated from cells derived from untreated vs. CTX treated Arf−/−Ph+ B-ALL-bearing mice on alemtuzumab-dependent phagocytosis of hMB cells. (C) A graph showing the effect of conditioned media generated from cells derived from untreated or DOX or CTX-treated Eμ-Myc/Arf−/− lymphoma-bearing mice on alemtuzumab-dependent phagocytosis of hMB cells. (D) A graph showing the relative level of alemtuzumab-mediated cell killing by human primary monocytes in the presence of conditioned media derived from control or CTX-treated leukemia cells. (E) A graph showing the level of alemtuzumab-mediated leukemia cell lysis, as determined by a europium-release assay, using primary human NK-cells from healthy donors. (F) A bar graph showing the level of human monocyte ADCC in the presence of PGE2, IL8, CCL4, VEGF, and TNFα. ( G) A graph showing the response of patient-derived B-ALL xenografts in NSG mice to treatment with rituximab (3×10mg/kg), total body irradiation (5Gy) CTX (2×100 mg/kg), or their respective combinations, as indicated. (H) A graph showing the level of rituximab-mediated ADCC following exposure of leukemia cells to conditioned media generated from tumor cells isolated from untreated, irradiated (5 Gy) and CTX -treated (100mg/kg) primary human patient B-ALL xenografted mice. Mafosfamide and 4-OH-CTX were used to treat cells ex vivo for 6h, and conditioned media was obtained after 24h of subsequent culture. Untreated leukemia cells served as target cells for Rituximab-mediated ADCC. For all graphs, * = p<0.05, ** = p<0.01 and *** = p<0.001.