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. 2014 Mar 11;11:22. doi: 10.1186/1742-4690-11-22

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Copyright © 2014 Tsai et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Phylogenetic tree analyses of T/F env from R5 SHIV_SF162P3N-ivg infected macaques. (A) Neighbor-joining tree of env V3-V5 sequences from each of the TV (green circle), CP (blue circle) and RP (red circle) macaque is shown. A total of 485 env V3-V5 sequences were analyzed (an average of 35 sequences per animal; range, 19 to 59). (B) Comparison of env V3-V5 sequences of GR56 and GH62 T/F viruses obtained by conventional PCR/cloning (●) and SGA (▲). Sequences are rooted to SF162.