Figure 4.

Assessment of seed basal heat tolerance in different Arabidopsis thaliana ecotypes. (A-D) Seeds were subjected to a 50°C heat shock for 60 min. (A) Radicle emergence and (B) green cotyledon emergence during the 12 days post-heat shock period. (C) Germination levels (radicle emergence parameter) after 12 days. (D) Morphology of 12-day-old seedlings. Percentages were determined in relation to the total number of sown seeds. Error bars represent SEM (n = 7 plates with ~25 seedlings each). In C, letters represent statistically different genotypes (One-way ANOVA with Tukey’s multiple comparison test for all pairs of columns; P < 0.05).