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. 2014 Mar 10;10:7. doi: 10.1186/1746-4811-10-7

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Copyright © 2014 Silva-Correia et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Assessment of seed basal heat tolerance in different Arabidopsis thaliana functional mutant genotypes. (A-D) Seeds were subjected to a 50°C heat shock for 60 min. (A) Radicle emergence and (B) green cotyledon emergence during the 12 day post-heat shock period. (C) Germination levels (radicle emergence parameter) after 12 days. (D) Morphology of 12-day-old seedlings. Percentages were determined in relation to the total number of sown seeds. Error bars represent SEM (n = 7 plates with ~25 seedlings each). Asterisks represent mutant genotypes with statistically significant differences in relation to the wild-type Col (Student t-test; ns, non-significant; ***, P < 0.001).