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Epithelial-mesenchymal transition in the present case. Immunohistochemistry for transcriptional factors, Slug (A) and Twist (B). Nuclear staining for Slug and Twist was observed in the neoplastic cells. Immunohistochemistry for mesenchymal markers, matrix metalloproteinase-2 (C) and -9 (D). Both matrix metalloproteinase-2 (C) and -9 (D) are expressed in the tumor cells.
