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. 2014 Mar 7;7:16. doi: 10.1186/1756-6606-7-16

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Copyright © 2014 Sindreu et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Synaptic targeting of ZnT1. (A) Bright field immunostaining of ZnT1 in mouse CA1 region. Neuronal perikarya and apical dendrites (arrowheads) were labeled. s.o. stratum oriens; s.p. stratum piramidale; s.r. stratum radiatum. Scale bar, 100 μm. (B) Confocal images of double stained dendrites of cultured pyramidal neurons. Scale bar, 5 μm. (C) Left, western blot for ZnT1 in nuclear (N) and cytoplasmic (C) extracts (20 μg per lane) from adult hippocampus. MW was assessed with biotinylated protein ladder. Right, Synaptic and extrasynaptic fractions (10 μg per lane) were probed for ZnT1, stripped, and reprobed for actin and PSD-95.