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Selenite reduction assay carried out on protein fractions (shown at the top) and on supernatant, boiled and not boiled, (shown at the bottom) of SeITE01 liquid cultures. All tests were done in duplicate (indicated by roman numbers), with addition of 2.0 mM SeO32− and 2.0 mM NADH. Three negative controls were set up: without protein fractions or supernatant, without selenite, without NADH.
