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. 2014 Feb 27;15(3):3560–3579. doi: 10.3390/ijms15033560

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — (A) Western blot VCAM-1 protein expression in NMuMG cells transfected with the pBabe-eGFP or the pBabe-VCAM-1 plasmid. β-actin results indicate similar sample loads; (B) a, Transwell migration analysis of NMuMG cells (control and VCAM-1 over-expression) towards 10% FBS + insulin. Photo images are representative fields of the migration of VCAM-1 over-expressing cells and the control group after crystal violet dye staining; b, The cell numbers of migrated NMuMG (eGFP control) and VCAM-1 NMuMG cells from the upper insert to the bottom surface of the lower well under a light micropore (100×) after overnight incubation; c, Migrated NMuMG VCAM-1 cells in the lower wells showed high clonogenic ability after 2 weeks of incubation; (C) EMT-related protein expression in eGFP control and VCAM-1 over-expressing NMuMG cells was determined by Western blot analysis.