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. 2014 Feb 27;15(3):3560–3579. doi: 10.3390/ijms15033560

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 4. — (A) a, Western blot analysis of MDAMB231 VCAM-1 siRNA cells showed reduced VCAM-1 expression after IL-6 treatment compared with control cells; b, VCAM-1 siRNA knockdown blocks TGF-β1 or IL6-induced MDAMB231 cell migration and colony formation. The lower chamber was filled with 2 mL regular medium (normal) or contained TGFβ1 (5 ng/mL) or IL-6 (1 ng/mL) as a chemoattractant; c, Representative photos showed the influence of VCAM-1 siRNA on the number of colony-forming MDAMB231 cells as evaluated by clonogenic assay; (B) The inhibition of migration and colony formation by VCAM-1 knockdown was confirmed by shRNA knockdown stable clones. a,b, similar results confirmed the influence of VCAM-1 shRNA knockdown on the migratory ability and number of colony-forming MDAMB231 cells. Columns, mean of triplicate samples; bars, SE; p-value was determined by student’s t-test (* p < 0.01).