Figure 5.

(A) The level of VCAM-1 protein expression in stable transfection clones was determined by western blotting; (B) Tumors were established using MDAMB231 eGFP and VCAM-1 shRNA knockdown clones implanted by s.c. injection and analyzed after eight weeks. Western blot analysis was used to confirm VCAM-1 knockdown efficacy in xenograft tumors (upper insert pictures); (C) Tumor weights were measured after autopsy and tumor volumes were calculated using the formula 4/3πr₁²r₂ (r₁ < r₂) as described in Materials and Methods. Mean ± SE (n = 6). * p < 0.01.