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. 2014 Mar 4;15(3):3871–3888. doi: 10.3390/ijms15033871

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 1. — Characterization of GmBRI1 protein. (a) Multiple alignment of the deduced GmBRI1 protein with brassinosteroid insensitive (BRIs) from various species (At, Arabidopsis; Bn, Brassica napus; Mt, Medicago truncatula; Ps, Pisum Sativum; Tc, Theobroma cacao; and Os, Oryza sativa). The predicted functional domains are marked in the relative positions. The amino acid sequences were obtained from the NCBI protein database. The amino acid sequences that are identical in all seven molecules are shaded black, while sequences that are conserved in four to six species are shaded gray; and (b) Phylogenetic analysis of GmBRI1 and homologs from other organisms. A bootstrap consensus tree was generated using the neighbor-joining method in MEGA 5.02. The bootstrap values from 1000 bootstrap replicates are shown next to the branches. The accession numbers are as follows: GmBRI1, XP_003526839; PsBRI1, BAC99050.1; MtBRI1, XP_003602504.1; TcBRI1, EOX92323.1; BnBRI1, AFU83229.1; AtBRI1, AAC49810; NbBRI1, ABO27628.1; StBRI1, ABO27627.1; SlBRI1, AAN85409.1; OsBRI1, NP_001044077.1.

Figure 1. — Characterization of GmBRI1 protein. (a) Multiple alignment of the deduced GmBRI1 protein with brassinosteroid insensitive (BRIs) from various species (At, Arabidopsis; Bn, Brassica napus; Mt, Medicago truncatula; Ps, Pisum Sativum; Tc, Theobroma cacao; and Os, Oryza sativa). The predicted functional domains are marked in the relative positions. The amino acid sequences were obtained from the NCBI protein database. The amino acid sequences that are identical in all seven molecules are shaded black, while sequences that are conserved in four to six species are shaded gray; and (b) Phylogenetic analysis of GmBRI1 and homologs from other organisms. A bootstrap consensus tree was generated using the neighbor-joining method in MEGA 5.02. The bootstrap values from 1000 bootstrap replicates are shown next to the branches. The accession numbers are as follows: GmBRI1, XP_003526839; PsBRI1, BAC99050.1; MtBRI1, XP_003602504.1; TcBRI1, EOX92323.1; BnBRI1, AFU83229.1; AtBRI1, AAC49810; NbBRI1, ABO27628.1; StBRI1, ABO27627.1; SlBRI1, AAN85409.1; OsBRI1, NP_001044077.1.